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Hiscript III Reverse Transcriptase

货  号:
R302-01
价  格:
¥
1000.00
数  量
-
+
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COA报告

HiScript® III Reverse Transcriptase

更高效的第三代逆转录酶

广泛的模板兼容性:可兼容动物、植物、病毒等多种模板

超强的杂质耐受度:对于常见杂质(乙醇、异丙醇、水平衡酚、异硫氰酸胍、腐殖酸)均具有超强的耐受度

卓越的逆转录效率:逆转录效率高于二代产品

以1μg HeLa细胞RNA为模板,按照R302说明书推荐体系进行逆转录,以1μl cDNA为模板,用2 ×Vazyme Lamp® Master Mix (Dye Plus)(Vazyme #P312)进行长片段扩增,将得到的产物进行琼脂糖凝胶电泳,结果如图所示:扩增条带单一且产量高。

长片段扩增电泳图

注:M:DL15000 Marker(Vazyme #MD103)

HiScript® III Reverse Transcriptase是HiScript® II Reverse Transcriptase的升级版本,37℃条件下即可进行高效率的逆转录反应。HiScript® III Reverse Transcriptase依旧保留二代产品HiScript® II Reverse Transcriptase的热稳定性,对于复杂二级结构的RNA,可将逆转录温度提高至50~55℃,避免RNA复杂二级结构对cDNA合成的抑制,可有效合成高质量的cDNA。除此之外,本产品仍具有优越的持续合成能力和超强的杂质耐受度。

组分

R302-01 (10000 U)

5 × HiScript®III Buffer

500 μl

HiScript®III Reverse Transcriptase (200 U/μl)

50 μl

- 30℃~- 15℃保存。

未找到相应参数组,请于后台属性模板中添加

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暂未实现,敬请期待
暂未实现,敬请期待