购物车中还没有商品,赶紧选购吧!

产品订购:sales@vazyme.com

技术支持:support@vazyme.com

在线
客服

在线客服服务时间:8:30-17:30

客服
热线

400-600-9335
客服服务热线

关注
微信

诺唯赞生物

关注
微信

诺唯赞资讯

诺唯赞资讯

地 址:江苏省南京经济技术开发区科创路红枫科技园C2栋   电话:400-600-9335    邮箱:sales@vazyme.com

版权所有-南京诺唯赞生物科技有限公司   备案号:苏ICP备12014779号-1   友情链接:南京诺唯赞医疗科技有限公司

诺唯赞生物

南京诺唯赞生物科技有限公司

>
>
>
>
HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper)
浏览量:

HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper)

货  号:
R312-01/02
价  格:
¥
1250.00
数  量
-
+
没有此类产品
立即购买
加入购物车
产品详情
FAQ
文献引用
COA报告

HiScript® III 1st Strand cDNA Synthesis Kit (+gDNA wiper)

第三代高效cDNA 一链合成试剂盒(去基因组)

基于更高效的HiScript®III Reverse Transcriptase:逆转录效率高于二代产品

灵活选择引物: 针对不同实验设计可灵活使用不同类型的逆转录引物

gDNA wiper Mix可迅速彻底去除基因组污染:保证后续结果更加可靠,并可简化qPCR引物设计,无需跨内含子设计引物

1.后续实验为PCR

以1μg  HeLa细胞RNA为模板,按照R302说明书推荐体系进行逆转录,以1μl cDNA为模板,用2 ×Vazyme Lamp® Master Mix (Dye Plus)(Vazyme #P312)进行长片段扩增,将得到的产物进行琼脂糖凝胶电泳,结果如图1所示:扩增条带单一且产量高

图1.长片段扩增电泳图

注:M:DL15000 Marker(Vazyme #MD103)

2.后续实验为qPCR

以100 ng HeLa 细胞 RNA为模板,使用 HiScript® III 1st Strand cDNA Synthesis Kit (+gDNA wiper)(Vazyme #R312) 以及市售其他逆转录产品 (分别来自Supplier Ta、Th1、Th2、N、P、R、B、Tr、Ti),按照各自说明书推荐程序进行逆转录反应。将得到的 cDNA 进行多个基因的 qPCR定量分析。以不同公司产品为横坐标,△CT (△CT = Vazyme #R312 cDNA扩增CT值–各公司逆转录产品 cDNA 扩增CT值)为纵坐标绘图。从图中可以看出,除一些体系Th1△CT>0外,其余公司逆转录产品均△CT<0,说明 Vazyme #R312逆转录效率优于Ta、Th2、N、P、R、B、Tr、Ti 相关逆转录产品。

HiScript® III 1st Strand cDNA Synthesis Kit (+gDNA wiper)是HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper)的升级版本,包含新一代逆转录酶HiScript® III Reverse Transcriptase和针对逆转录优化的最适Buffer,进一步提高了一链合成的效率。该试剂盒中的5 × gDNAwiper Mix可在42℃,2 min条件下快速去除基因组DNA污染,保证后续结果更加可靠,并可简化qPCR引物设计,无需跨内含子设计引物。试剂盒中包含单组分逆转录引物Oligo (dT)20VN和Random hexamers,用户可根据需要,灵活选择逆转录引物进行后续实验。本试剂盒既可合成用于克隆等下游实验的全长cDNA (可达20 kb),亦可合成高度均一的用于qPCR定量的cDNA。

组分

R312-01 (50 rxn)

R312-02 (100 rxn)

RNase-free ddH2O

1 ml

1 ml

5 × gDNA wiper Mix

100 μl

200 μl

10 × RT Mixa

100 μl

200 μl

HiScript®III Enzyme Mixb

100 μl

200 μl

Oligo (dT) 20VN

50 μl

100 μl

Random hexamers

50 μl

100 μl

a. 包含dNTP

b. 包含RNase inhibitor

- 30℃~- 15℃保存

未找到相应参数组,请于后台属性模板中添加

Liu X ,Wei W,Li X , et al. (2017). BMI1 and MEL18 Promote Colitis-Associated Cancer in Mice via REG3B and STAT3.Gastroenterology.IF:18.392

Ju J, Chen A, Deng Y, et al. (2017).NatD promotes lung cancer progression by preventing histone H4 serine phosphorylation to activate Slug expression.[J]. Nature Communications, 2017, 8(1):928.IF:12.124

Ding W, Liu WQ, Jia Y, et al. (2016). Biosynthetic investigation of phomopsins reveals a widespread pathway for ribosomal natural products in Ascomycetes.PNAS, 113(13):3521-3526. IF:9.674

Wang H, Liang L, Dong Q,et al.(2018)Long noncoding RNA miR503HG, a prognostic indicator, inhibits tumor metastasis by regulating the HNRNPA2B1/NF-κB pathway in hepatocellular carcinoma. Theranostics.IF:8.172

Zhang B, Wang M, Gong A, et al. (2015). HucMSC-exosome mediated -Wnt4 signaling is required for cutaneous wound healing. Stem Cells, 33(7):2158-2168.IF:7.133

Zhang Y, Du J, Zheng J, et al. (2015). EGF-reduced Wnt5a transcription induces epithelial-mesenchymal transition via Arf6-ERK signaling in gastric cancercells. Oncotarget. IF:6.627

Balamurugan S, Wang X, Wang H L, et al. (2017). Occurrence of plastidial triacylglycerol synthesis and the potential regulatory role of AGPAT in the model diatom Phaeodactylumtricornutum[J]. Biotechnology for Biofuels, 2017, 10(1):97.IF:6.444

Wang Y, Huang Y, Xu Y, et al. (2017).A Dual AMPK/Nrf2 Activator Reduces Brain Inflammation after Stroke by Enhancing Microglia M2 Polarization[J]. Antioxidants & Redox Signaling, 2017.IF:6.337

Gu J, Wang D, Zhang J, et al. (2016). GFRalpha2 prompts cell growth and chemoresistance through down-regulating tumor suppressor gene PTEN via Mir-17-5p in pancreatic cancer. Cancer Lett, 380(2), 434-441. IF:5.992

Wang Y, Ruan W, Mi J,et al. (2017).Balasubramide derivative 3C modulates microglia activation via CaMKKβ-dependent AMPK/PGC-1α pathway in neuroinflammatory conditions[J]. Brain Behavior & Immunity, 2017.IF:5.964

Si Z, Liu H, Zhu J, et al.2018Mutation ofSELF-PRUNINGhomologs in cotton promotes short-branching plant architecture:[J]. Journal of Experimental Botany, 2018, 69(10):2543-2553.IF5.83

Zhu J, Chen J, Gao F, et al. 2017Rapid mapping and cloning of the virescent-1 gene in cotton by bulked segregant analysis-next generation sequencing and virus-induced gene silencing strategies.[J]. Journal of Experimental Botany, 2017, 68(15):4125.IF5.83

暂未实现,敬请期待
暂未实现,敬请期待