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TruePrep Index Kit V2 for Illumina

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    TruePrep Index Kit V2 for Illumina-V21.2.pdf

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TruePrep Index Kit V2 for Illumina

TruePrep Index Kit V2 for Illumina 8种N5XX, 12种N7XX,组合使用可制备96种不同的双端Index文库 每批次严格质量控制和功能验证 TruePrep Index Kit V2 for Illumina是TruePrep DNA Library Prep Kit V2 for Illumina配套试剂盒。其中包含8种N5XX以及12种N7XX,可用于96种不同的双端Index文库制备。试剂盒中提供的所有试剂都经过严格的质量控制和功能验证,较大程度上保证了文库构建的稳定性和重复性。 所有组分-30 ~ -15℃保存,≤0℃运输
价格:
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1400.0
浏览量:
5070
货号:
TD202
所属分类
DNA建库接头
数量:
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库存:
9999
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TruePrep Index Kit V2 for Illumina

Illumina 平台双端Index标记文库的制备

8种N5XX, 12种N7XX,组合使用可制备96种不同的双端Index文库

每批次严格质量控制和功能验证

TruePrep Index Kit V2 for Illumina是TruePrep DNA Library Prep Kit V2 for Illumina配套试剂盒。其中包含8种N5XX以及12种N7XX,可用于96种不同的双端Index文库制备。试剂盒中提供的所有试剂都经过严格的质量控制和功能验证,较大程度上保证了文库构建的稳定性和重复性。

所有组分-30 ~ -15℃保存,≤0℃运输

关键词:
TruePrep Index Kit V2 for Illumina®
TD202
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Q1 :TruePrep Index Kit V2 for Illumina® (Vazyme #TD202)能提供多少index组合?

A1:最多提供384种index组合,其中包含8种N5XX和12种N7XX。

 

Q2: 使用TD501/502/503构建DNA文库时,能否用VAHTSTM DNA 接头(Vazyme # N321/N322)替代TruePrep 接头(Vazyme #TD202)?

A2:不可以。转座酶建库接头连接的原理与机械打断建库不同。

 

Q3 :转座酶建库与普通DNA建库后上机测序有区别吗?

A3:有区别。转座酶接头有19bp的核心激活序列,在测序过程中大部分Illumina测序平台是可以兼容的,建议老师测序前咨询具体的情况。

 

Si J, Shi X, Sun S, et al. Hematopoietic Progenitor Kinase1 (HPK1) Mediates T Cell Dysfunction and Is a Druggable Target for T Cell-Based Immunotherapies. Cancer Cell. 2020, 38(4): 551-566.e11. PMID:32860752 (IF:26.602)

Sun S, Jiang Y, Zhang Q, et al. Znhit1 controls meiotic initiation in male germ cells by coordinating with Stra8 to activate meiotic gene expression. Dev Cell. 2022, 57(7): 901-913. PMID:35413238 (IF:12.27)

Yuan S, Huang T, Bao Z, et al. The histone modification reader ZCWPW1 promotes double?strand break repair by regulating cross?talk of histone modifications and chromatin accessibility at meiotic hotspots. Genome Biol. 2022, 23(1): 187. PMID:36068616 (IF:17.906)

Ding Y, Wang W, Ma D, et al. Smarca5-mediated epigenetic programming facilitates fetal HSPC development in vertebrates. Blood. 2021, 137(2): 190-202. PMID:32756943 (IF:17.794)

Chen X, Wang P, Qiu H, et al. Integrative epigenomic and transcriptomic analysis reveals the requirement of JUNB for hematopoietic fate induction. Nat Commun. 2022, 13(1): 3131. PMID:35668082 (IF:14.919)

Yuan J, Zhu Q, Zhang X, et al. Ezh2 competes with p53 to license lncRNA Neat1 transcription for inflammasome activation. Cell Death Differ. 2022, . PMID:35568718 (IF:15.828)

Wang H, Gong P, Chen T, et al. Colorectal Cancer Stem Cell States Uncovered by Simultaneous Single-Cell Analysis of Transcriptome and Telomeres. Adv Sci (Weinh). 2021, 8(8): 2004320. PMID:33898197 (IF:15.804)

Gao J, Zhang K, Cheng Y, et al. A robust mechanism for resetting juvenility during each generation in Arabidopsis. Nat Plants. 2022, 8(3): 257-268. PMID:35318444 (IF:15.793)

Chen X, Wang P, Qiu H, et al. Integrative epigenomic and transcriptomic analysis reveals the requirement of JUNB for hematopoietic fate induction. Nat Commun. 2022, 13(1): 3131. PMID:35668082 (IF:14.919)

Wu X, Zhang H, Zhang B, et al. Methylome inheritance and enhancer dememorization reset an epigenetic gate safeguarding embryonic programs. Sci Adv. 2021, 7(52): eabl3858. PMID:34936444 (IF:14.143)

Yin Y, Yang X, Wu S, et al. Jmjd1c demethylates STAT3 to restrain plasma cell differentiation and rheumatoid arthritis. Nat Immunol. 2022, 23(9): 1342-1354. PMID:35995859 (IF:31.25)

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